Tasco 750 Monocular Microscope Logo
Posted on Dec 30, 2007

Microscopy; Relatively large spots on unknown surface obstructing view

Hey there,

I have a infinity light microscope and when I raise the condenser (or adjust the focus) to a certain height many spots with a bluish/red tinge appear in the field of view. I have turned the eyepieces, entire condenser (with attached filter), objectives and the spots remain stationary. I can't be sure whether it is dust or not, and I'm concerned they may be scratches, although the microscope is brand new. What do scratches/lens imperfections look like under the microscope? Also is there any other way of locating the problem?

2 Answers

Anonymous

Level 1:

An expert who has achieved level 1.

Problem Solver:

An expert who has answered 5 questions.

  • Contributor 7 Answers
  • Posted on Feb 20, 2008
Anonymous
Contributor
Level 1:

An expert who has achieved level 1.

Problem Solver:

An expert who has answered 5 questions.

Joined: Feb 13, 2008
Answers
7
Questions
0
Helped
2155
Points
5

HELLO..

There is five part in microscope

1. Eye piece

2. Binocular tube

3.Objective

4. magnification changer or joom accembaley

5. illumination segment

Try to make it out which part is gives a effect to your vision.

as per my suggesition illumination asembely you just clean it first.

keep in touch

regards

INDRAVIJAY

Anonymous

Level 1:

An expert who has achieved level 1.

Hot-Shot:

An expert who has answered 20 questions.

Corporal:

An expert that has over 10 points.

Mayor:

An expert whose answer got voted for 2 times.

  • Contributor 30 Answers
  • Posted on Mar 10, 2008
Anonymous
Contributor
Level 1:

An expert who has achieved level 1.

Hot-Shot:

An expert who has answered 20 questions.

Corporal:

An expert that has over 10 points.

Mayor:

An expert whose answer got voted for 2 times.

Joined: Mar 07, 2008
Answers
30
Questions
2
Helped
19530
Points
54

Hey there to you too!

I suggest you don't have a problem at all. You are focusing on a ground glass filter somewhere in the light train. This serves the purpose of diffusing the light to give even illumination across the field of view.
When setting up, firstly use low power and a simple, easy specimen. Focus on this then rack up the condenser untill you see your annoying speckles. Rack up the condenser a little more untill the speckles just go out of focus. This is the position where your condenser should be for normal use.
Setting up the light source is the most important pre requasit for a good image, and there is a lot more to it than what I have already mentioned.
If I can be of any further help......Don't hesitate

Cheers and good hunting...Dave

Ad

Add Your Answer

×

Uploading: 0%

my-video-file.mp4

Complete. Click "Add" to insert your video. Add

×

Loading...
Loading...

Related Questions:

0helpful
1answer

I have the 75X-900X specialty microscope and it came with methyl orange, gum, and methylene blue. I am not sure what to use these three items on. The instructions say nothing about them.

the methyl orange and methylene blue are stains for use on thin
plant sections to show cell structures.
the gum is to attach coverslips to slides.
Try Googling microscopy there is one particularly good site
something like microscopical discussions but you could start with
microscopy-uk.org.uk.
Have fun
0helpful
1answer

Pinion gear for raising & lowering condenser tray is broken need part #

In the Optiphot there are two small grub screws under the left hand side of the condenser column. Once loosened the metal housing of the pinion can by pulled or pushed out and the new one inserted. You may need a small mirror to see the screws due to their hidden position. You can then replace the pinion in which the black nylon gear will have broken
0helpful
1answer

Olympus microscope BX41

http://www.microscopy.olympus.eu/microscopes/39_manuals.cfm?prodID=P_T00004&cm_mc_uid=24669203090613385112351&cm_mc_sid_90274701=1338511235
0helpful
1answer

I have a microscope from National (DC3-163). How can I hook this microscope up to my MAcBook Pro. I only have Motic software that's compatible with Windows. Kris

I did a bit of research, and it looks like the only way that you can use the "Mac OSX compatible" microscope, is using it the same way one would plug in a webcam via USB. Essentially, you can plug in the Microscope, open up the Image Capture Utility, PhotoBooth, or Quicktime/iMovie (I'd recommend one of these two), make sure you have the Capture Device set to the microscope and you'll be able to "project" the image that you are seeing through the microscope onto the computer.
The Motic software does not have a counterpart for the Apple OS, and can only be used if you have a Windows BootCamp Partition, or if you have a Parallels installation of Windows that can use the Motic Software. There are other pieces of software out there that can utilize microscopes, but may not have all the same features as the Motic Bundled software. One that I found that may be of some use is called miXscope. It can be found here: http://www.edhsw.com/mixscope/ , and it is relatively inexpensive in terms of licensing.
This may also be of some help for selecting compatible microscopes in the future: http://www.apple.com/science/solutions/microscopy.html
Hope that helps!
1helpful
1answer

I'm using the Nikon ECLIPSE e800 microscope and for some reason there is a shadow on half of my slide. Also, I can usually find and take pictures of slides with the lamp adjusted at about 5 or so but...

There are a few things that could cause a shadow in the field of view. First if the condenser is not centered, this can create a crescent shaped shadow and rob your image of light. Center the condenser and raise it to within about a 16th of an inch from the top of the stage.
Second, the slider on the head which diverts light from the eyepieces to the camera must be fully engaged to the "camera" position
Third, make sure that the diaphragm on the condenser is not closed too far. There is an adjustment ring for this. Set it to 70-80% of the numerical aperture of the objective you are using.
Fourth: if there is a "swing out" condenser lens you will need it completely in or out depending on the objective you are using. for 2x-5x you need the swing out condenser lens completely out of the light path. For objectives 10x and up you need the swing out condenser lens completely in and centered in the light path.
If these things are properly adjusted you should not have a shadow in the field of view. If you still have a problem, let me know.
0helpful
1answer

WE ARE TRYING TO TAKE PICTURES WITH OUR MICROSCOPE CAN YOU HELP?

You will need a Trinocular head and a digital microscopy camera. Contact Leica at their website. A Google search will get you well on your way.
1helpful
1answer

Radical Microscope for Christmas...no user manual

You don't need a specific microscope manual for that microscope, any old microscopy guide will do as at that level, all microscopes work pretty much the same way. Enjoy.
0helpful
1answer

Image rings when using Coolpix for microscopy

Under some circumstances when using the Coolpix 4500 attached to a microscope concentric rings are visible as in the below. The COOLPIX 4500 aspherical lens surface is ground to create the ideal curves to gather rays at the focal point with an extremely high accuracy. Such minute lines are not visible in the image taken with a camera alone, however if the camera is used with a microscope, the lines become visible as the depth of field is increased due to the combined F numbers of the camera lens and microscope lenses.
Not finding what you are looking for?

291 views

Ask a Question

Usually answered in minutes!

Top Tasco Science & Laboratory Experts

ADMIN Andrew
ADMIN Andrew

Level 3 Expert

66831 Answers

Don A
Don A

Level 3 Expert

4408 Answers

Collins Mccoy
Collins Mccoy

Level 2 Expert

58 Answers

Are you a Tasco Science and Laboratory Expert? Answer questions, earn points and help others

Answer questions

Manuals & User Guides

Loading...