It appears that some water got into our 40x objective. When we took the objective off and looked through it, we can see an air bubble. How do we get rid of it? Thanks.

The water container has a dime looking ring on the side of it. The side that meets the machine.. There is a sensor on the machine that lines up with that metal ring on water container. This is a photo eye. What is happening is air bubbles are building up on the wall of the water container. Kind of like a carbonated drink. the eye is getting interrupted by small air bubbles coming in between the metal ring and photo eye on the machine. So tap on container like you would a soft drink. Slowly remove the container and you will see a couple air bubbles resting on sensor ring. IT THINKS ITS EMPTY. It usually happens when coffee pot sets for awhile. Air bubbles collect on the sides.

Do you have a water separator attached?

The oil goes between the cover slip of the slide and the objective tip. It can also help to put some between the condenser lens and the bottom of the slide. Be careful when using oil, as when rotating to lower magnifications, it is very possible for the 40x to get oil on it. The 100x is sealed to protect it, but the 40x is not. If oil gets on the 40x, it can seep up inside and destroy the lens.

It is also a very good practice to clean both the 100x and 40x after each use. If the oil sits on even the sealed 100x objective for any long extent of time, it will start to break down the silicon seal, and you can potentially have the same problem as with the 40x, where the objective acts like a wick of a candle and sucks up the oil, thereby destroying the objective.

Since you need to use oil at 100x, there is probably a good chance that there is oil on both the 100x and 40x objective. I see this a lot in my work. Since the working distance of the 40x is so close to the slide, what often times will happen is that it will accidentally get dragged through the oil while rotating to lower magnifications. The 100x is sealed to protect it against the oil, but the 40x is not, and if the oil sits on the objective for any length of time, the objective will act like a wick of a candle and **** the oil up inside of it. At that point it is more economical to buy a new object. The same thing can happen with the 100x, but it takes much longer for it to happen. Basically the seal around the objective tip is just latex chalking, and the oil will break it down over time, thereby causing the same affect as with the 40x.

You can try to clean them. If you are lucky the oil is only on the outside surface of the objective. Try using a que-tip and isophrapoyl alcohol to clean it off.

You can send the lens to Delta Optical Instruments, Inc.They can determine if your EF Plan 40x objective lens is repairable.The will give you an estimate prior to work or suggest a replacement if it cannot be repaired.email them at:  [email protected]

probably a "O" ring as the seal
if the pump is doing what it is supposed to be doing and everything is normal. I would not stress about air under the cover as water contains air in suspension ( how fish get their oxygen to live) and from the turbulent action of the pump impeller , some of that air will be released and collected at the highest point of the flow line

As the pump is running try spraying all the fittings with soapy water and look for bubbles. Hope this helps. Good luck.

I had the same,drove me nuts,made sure of fuel and spark(about 10 times),all good.anyway after it stopped looked under bonnet to find petrol coming out of carb,(didn't notice this before because the petrol had evaporated by the time i looked).took carb apart ,cleaned and checked jets,all resonable.Then i looked at the float seemed good but checked by putting into boiling water only to find small bubbles coming out of the float !!!!! thus float not floating right, too much petrol and flooding and then stopping.But let it cool down and everything sorts itself out and your good for another 10 minutes.You can check for air leeks by spraying wd40 around suspected areas and looking for bubbles.am no expert but hope this helps.
In short air leaks bad. Float not floating bad.

check for air bubbles in the system also check the pressure cap and have it tested also squeeze the upper radiator hose together and if it doesn't open up in your hand right away the water pump could be weak work on getting the air bubbles out first before going to the water pump

Hi there,

Seing bacteria isn't just about magnification, many
are transparent and need to be stained. Even at X1000, you will see little detail, but can make approximations of shape etc. Here is a good starting point for staining bacteria.
With a toothpick scrape a little plaque from your teeth (size of a pinhead is plenty). Put this in the centre of a slide with 1 drop of water and mix thoroughly. Allow this to dry then pass the sample through a flame three or four times (hot, but not hot enough to burn fingers) Stain for five mins using either Methylene Blue or Eosin. If you don’t have these, Blue or Red fountain pen ink will do for starters. Rinse off excess stain with very slow running water. Blot dry and observe at X400. If you have an oil immersion lens, you must use a cover slip and mount your specimen in balsam first.
If I can be of any further help, please don’t hesitate to ask.
Cheers…..Dave