A couple of possible reasons:
You are trying to take the shot with the lens too close to the subject. If you attempt to take a shot with AF on while too close then the camera won't take the photo. Try backing up from the subject a bit.
Which camera mode are you in? If you are using the green box (automatic) mode then in very low-light settings the camera may refuse to take the photo.
The AF on your lens might be broken. Try using a different lens (if you have one available). Do other lenses have the same problem?
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